Clinical Pharmacy and Pharmaceutical Care (Ishida Lab.)

Dr. Shiro Ishida

Research projects
Research of our group is focused on the study of the adjsutment, assessment and establishment of drug usage and infomatic technology of drug interaction. Through studying, we expect to better understand the assessment of drug action for basic aspectd and clinical usage.

Basical assessment derived from the drug actions
-pharmacodynamics of drug-drug, drug-chemicals, drug-metal and drug-food
-action and mode of intracellular zinc-
-disorder of feeding and swallowing n the aging subjects and its disease-
-analysis of drug interaction using simple emulsion method-

Clinical assessment derived from the drug actions
-analysis of injectable surfactant on the cytotoxicity-
-propofol, an anesthetic possessing neuroprotective action against oxidative
stress, promotes the process of cells death induced by H2O2 in rat thymocytes-
-synergic cytotoxic action induced by simultaneous application of zinc and antifungals
(clotrimazole etc.)-
-the interaction of zinc or these derivatives with oxidative stress-
-a new insight into toxicological characteristics of drug and supplement


  1. Oyama T M , Ishida  S, Okano Y, Seo H, and Oyama Y; Clioquinol-induce changes in the intracellular Zn2+ level in rat thymocytes. Toxicology in Vitro (2012) in press
  2. Yokoyama Y, Matsumoto K, Yamamoto H, Iguro Y, Imoto Y, Ikawa K, Morikawa N, Ishida S, Okano Y, Watanabe E, Shimodozono Y, Yamada K, Takeda Y;  Pharmacokinetics of ampicillin-sulbactam and the renal function-based optimization of dosing regimens for prophylaxis in patients undergoing cardiovascular surgery. Int J Antimicrobial Agents (2012) in press
  3. Jinnouchi O, Kuwahara T, Ishida S, Okano Y, Kasei Y, Kunitomo K, Takeda N; Anti-microbial and therapeutic effects of modified Burow’s solution on refractory otorrhea. Auris Nasus Larynx (2012) in press
  4. Oyama T M, Saito M, Yonezawa T, Okano Y, Oyama Y;  Nanomolar concentrations of zinc pyrithione increase cell susceptibility to oxidative stress induced by hydroge peroxide in rat thymocytes. Chemosphere (2012) 26 ,1-7
  5. Koizumi K, Kawanai T, Hashimoto E, Kanbara Y, Masuda T, Kanemaru K, Okano Y, Oyama Y; Cytometric analysis on cytotoxicity of curcumin on rat thymocytes : Proapoptosis and antiapoptotic actions of curcumin. Toxicol in Vitro (2011) 26, 1-6
  6. Kinazaki A, Chen H, Koizumi K, Kawanami T, Oyama T M, Satoh M, Ishida S, Okano Y, Oyama Y; Putative role of intracellular Zn release during oxidative stress : A trigger to restore cellular thiol content that is decreased by oxidative stress. J Physiol Sci (2011) 61, 403-409
  7. Oyama T M, Oyama K, Oyama T B, Ishida S, Okano Y, Oyama Y; Zinc at clinically-relevant concentrations potentiates the cytotoxicity of polysorbate80, a non-ionic surfactant.Toxicol in Vitro (2010) 24, 737- 744
  8. Matsui H, Oyama T M, Okano Y, Hashimoto E, Kawanai T, Oyama Y; Low micromolar zinc exerts cytotoxic action under H2O2-induced oxidative stress:Exessiveincrease in intracellular Zn2+ concentration. Toxicol (2010) 276, 27-32
  9. Demircioglu R I, Usta B, Muslu B, Sert H, Gozdemir M, Karabayirli S, Ishida S, Okano Y, Onodera K; Combination of small doses of subarachinoid morphine with systemic diclofenac improves analgesia during 48 hours after cesarean delivery. J. Hard Tissue Biol  (2010)19, 117-122
  10. Sakanashi Y, Oyama T M, Matsuo Y, Oyama T B, Nishimura Y, Ishida S, Imai S, Okano Y, Oyama Y; Zn2+ derived from cell preparation, partly attenuates Ca2+-dependent cell death induced by A23187, calcium ionopore in rat thymocytes. Toxicol. in Vitro (2009) 23, 338- 345